Resolution of Quadruplex Polymorphism by Size‐Exclusion Chromatography
نویسندگان
چکیده
منابع مشابه
Polymorphism and resolution of oncogene promoter quadruplex-forming sequences.
We report the separation of several quadruplex species formed by ten promoter sequences by Size Exclusion Chromatography (SEC). Modification at the 5' or 3' ends or in loop regions of quadruplex forming sequences has become the standard technique for dealing with quadruplex polymorphism. However, conformations produced employing this method or by other means of artificially shifting the equilib...
متن کاملResolution and characterization of the structural polymorphism of a single quadruplex-forming sequence
The remarkable structural polymorphism of quadruplex-forming sequences has been a considerable impediment in the elucidation of quadruplex folds. Sequence modifications have commonly been used to perturb and purportedly select a particular form out of the ensemble of folds for nuclear magnetic resonance (NMR) or X-ray crystallographic analysis. Here we report a simple chromatographic technique ...
متن کاملTopotecan enantio-specific resolution by liquid chromatography
Enantiomer specific method development is essential to ensure the safety and efficacy of the drug. Topotecan is the first topoisomerase inhibitor developed for ovarian cancer and lung cancer. Simple, rapid enantio specific chromatographic method was developed for R (-) enantiomer control in topotecan with immobilized chiral stationary phase (CSP). Chiral pak IA 250 mm long, 4.6 mm inner diamete...
متن کاملResolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography.
Bandeiraea simplicifolia I (BS I) isolectins, immobilized on Sepharose beads, specifically retarded low molecular weight ligands containing terminal cu-o-galactopyranosyl and 2acetamido-2-deoxy-a-D-galactopyranosyl residues. A BS I lectin-Sepharose column has been used to perform very efficient separations of structurally homologous sugar nucleotides and oligosaccharides. For example, UDP-gluco...
متن کاملResolution of intact phosphatidylinositols by argentation thin-layer chromatography.
An argentation thin-layer chromatographic method is described for the separation of intact phosphatidylinositols on the basis of the degree of unsaturation of their component fatty acids. The system is applicable for metabolic studies using radioactive precursors of phosphatidylinositol.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2011
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc1703s45